Influence of dietary Spirulina inclusion and lysozyme supplementation on the longissimus lumborum muscle proteome of newly weaned piglets

Arthrospira platensis (Spirulina) is a microalga with high content of crude protein. It has recalcitrant cell wall that limits the accessibility animal endogenous enzymes to its intracellular nutrients. Enzymatic supplementation aiming degrade walls could benefit microalgae digestibility. The objective this study was evaluate impact dietary Spirulina and lysozyme over muscle proteome piglets during post-weaning stage. Thirty were randomly distributed among three diets: control (no microalga), SP (10% Spirulina) + L +0.01% lysozyme). After 4 weeks, they sacrificed samples longissimus lumborum taken. analysed using Tandem Mass Tag (TMT)-based quantitative approach. A total 832 proteins identified. Three comparisons computed: vs Ctrl, Ctrl SP. They had ten, four twelve differentially abundant proteins. Glycogen metabolism nutrient reserves utilization are increased in piglets. Structural protein synthesis increased, causing higher energy requirements Our results demonstrate usefulness proteomics disclose effect microalgae, whilst unveiling putative mechanisms derived from supplementation. Data available via ProteomeXchange identifier PXD024083. Spirulina, microalga, an alternative conventional crops which enhance environmental sustainability production. Due wall, use requires additional measures prevent anti-nutritional effects on feeding period, endure stress. One such be CAZyme help digestion. Muscle provides insightful data enzyme activity piglet metabolism.